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Updated: Oct 10, 2025
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Potential effects of mung bean protein and a mung bean protein-polyphenol complex on oxidative stress levels and
Shu Zhang1,2, Yan-Tao Ma1, Yu-Chao Feng1
1College of Food, Heilongjiang Bayi Agricultural University, Xinfeng Lu 5, Daqing 163319, PR China. byndwcy@163.com.
Abstract:
This study investigated the effects of mung bean protein (MPI) and a MPI-polyphenol complex on oxidative stress levels and intestinal microflora in a D-galactose-induced aging mouse model. MPI and MPI-polyphenol complex intervention significantly increased activity of superoxide dismutase (SOD) and catalase and other antioxidant enzymes, improved the abundance and diversity of intestinal flora, and decreased the Firmicutes to Bacteroidetes ratio. Among them, the complex was more conducive to the improvement of the activity of antioxidant enzymes. The addition of MPI and the MPI-polyphenol complex can help the proliferation of Bacteroidetes, Bifidobacterium and Roseburia in the intestinal tract of aging mice, and inhibit the growth of Firmicutes and Ruminococcus, and the proliferation effect of the complex on Bifidobacterium was better than that of MPI. MPI significantly upregulated five pathways related to lipid and energy metabolism. Roseburia and Muribaculaceae were negatively correlated with malondialdehyde levels and positively correlated with SOD and other antioxidant enzyme indices. Our findings showed that MPI and MPI-polyphenol complexes can delay aging in mice by reducing oxidative damage and regulating intestinal flora. We also found a strong relationship between the abundance of intestinal flora and the levels of oxidative stress-related enzymes. This study provides theoretical support for the health and anti-aging benefits of mung bean food products.
Insights
Mung bean protein (MPI) and its polyphenol complex combat aging in mice by reducing oxidative stress and improving gut bacteria. The complex enhanced antioxidant enzyme activity and beneficial gut microbes like Bifidobacterium.
Area of Science:
- Nutrition Science
- Microbiology
- Gerontology
Background:
- Aging is associated with increased oxidative stress and detrimental changes in gut microbiota composition.
- Mung bean protein (MPI) is a plant-based protein source with potential health benefits.
- Polyphenols are known for their antioxidant properties.
Purpose of the Study:
- To investigate the anti-aging effects of mung bean protein (MPI) and an MPI-polyphenol complex in a D-galactose-induced aging mouse model.
- To evaluate the impact of MPI and its complex on oxidative stress markers and intestinal microflora.
- To explore the relationship between gut microbiota and oxidative stress in aging.
Main Methods:
- Administration of mung bean protein (MPI) and MPI-polyphenol complex to D-galactose-induced aging mice.
- Measurement of antioxidant enzyme activities, including superoxide dismutase (SOD) and catalase.
- Analysis of intestinal microflora composition and diversity using sequencing techniques.
- Assessment of lipid peroxidation marker malondialdehyde (MDA).
Main Results:
- Both MPI and the MPI-polyphenol complex significantly increased antioxidant enzyme activity and improved gut microbiota diversity.
- The MPI-polyphenol complex showed a more pronounced effect on antioxidant enzyme activity compared to MPI alone.
- Interventions decreased the Firmicutes to Bacteroidetes ratio and promoted the growth of beneficial bacteria like Bacteroidetes, Bifidobacterium, and Roseburia.
- MPI upregulated lipid and energy metabolism pathways. Roseburia and Muribaculaceae were positively correlated with antioxidant indices and negatively correlated with MDA.
Conclusions:
- Mung bean protein (MPI) and its polyphenol complex demonstrate anti-aging potential by mitigating oxidative damage and modulating gut microbiota in mice.
- The observed improvements in gut health and antioxidant capacity provide a mechanistic basis for the anti-aging effects.
- These findings support the use of mung bean-based food products for promoting health and combating aging.
