Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

13.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
13.7K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.0K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.0K
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

544
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
544
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

11.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
11.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Composition-dependent hydrogen oxidation activity of Pt-Cu nanoparticles prepared using boron-rich nanosheets.

Chemical communications (Cambridge, England)·2026
Same author

Mobile Protein Microboxes on Optical Fibers for Spatial Micromanipulation of Bacterial Microcolonies.

Analytical chemistry·2026
Same author

Dual-Functional Hydrogen-Bonded Organic Frameworks for the Detoxification and Capture of a Mustard Gas Simulant.

ACS applied materials & interfaces·2025
Same author

Scalable Templated Fabrication of Cu-based MOF on Textiles for Simultaneous Sensing, Filtration, and Detoxification of SO<sub>2</sub>.

Chem·2025
Same author

Defect-Driven Electrochemical Domain Modulation in Prussian Blue Revealed by Single-Entity Analysis.

Journal of the American Chemical Society·2025
Same author

Ternary Alloy Cu-Ru-Ir Nanocages for Acidic Oxygen Evolution Reaction.

ACS nano·2025

Related Experiment Video

Updated: Oct 10, 2025

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

7.1K

iR drop in scanning electrochemical cell microscopy.

Brandon Blount1, Gabriel Juarez1, Yufei Wang1

  • 1Department of Chemistry, The University of Texas at Austin, 105 E 24th St, Austin, TX 78712, USA. hren@utexas.edu.

Faraday Discussions
|December 8, 2021
PubMed
Summary

Accurate electrocatalysis research requires understanding the iR drop. This study presents methods to estimate and compensate for the iR drop in scanning electrochemical cell microscopy (SECCM) for precise local electrochemistry measurements.

More Related Videos

Scanning-probe Single-electron Capacitance Spectroscopy
10:53

Scanning-probe Single-electron Capacitance Spectroscopy

Published on: July 30, 2013

13.2K
Electron Channeling Contrast Imaging for Rapid III-V Heteroepitaxial Characterization
07:50

Electron Channeling Contrast Imaging for Rapid III-V Heteroepitaxial Characterization

Published on: July 17, 2015

11.2K

Related Experiment Videos

Last Updated: Oct 10, 2025

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

7.1K
Scanning-probe Single-electron Capacitance Spectroscopy
10:53

Scanning-probe Single-electron Capacitance Spectroscopy

Published on: July 30, 2013

13.2K
Electron Channeling Contrast Imaging for Rapid III-V Heteroepitaxial Characterization
07:50

Electron Channeling Contrast Imaging for Rapid III-V Heteroepitaxial Characterization

Published on: July 17, 2015

11.2K

Area of Science:

  • Electrochemistry
  • Materials Science
  • Nanotechnology

Background:

  • Scanning electrochemical cell microscopy (SECCM) is vital for mapping local electrochemistry and revealing electrode heterogeneity.
  • Accurate kinetic measurements in SECCM depend on precise assessment of the solution's ohmic potential drop (iR drop).

Purpose of the Study:

  • To assess and provide methods for estimating and compensating the iR drop in SECCM experiments.
  • To enable more accurate local electrochemistry measurements for understanding electrocatalysis.

Main Methods:

  • For single-barrel SECCM, iR drop estimation using solution conductivity and pipette geometry, or mass transfer limiting current.
  • For dual-barrel SECCM, direct measurement of solution resistance to compensate for iR drop and potential shifts.

Main Results:

  • Demonstrated methods for quantifying iR drop in both single- and dual-barrel SECCM configurations.
  • Validated approaches for accurate iR drop compensation, crucial for reliable kinetic data.

Conclusions:

  • The developed methods offer convenient and accurate ways to address the iR drop in SECCM.
  • Improved iR drop compensation enhances the reliability of local electrode kinetics measurements, aiding electrocatalysis mechanism determination.