DNase I-like activity and actin content in the liver of some vertebrates

Total actin content and F:G actin ratio were determined in the liver cytosol of fish, frogs and mouse by measurements of inhibition of exogenous crystalline bovine pancreatic DNase I. Endogenous DNase I-like activity, was found in all examined livers after electrophoresis in the presence of sodium dodecyl sulfate and subsequent enzyme renaturation. It is concluded that DNase I-like enzymes occur in the liver cytosol in a latent form, probably bound to cytoplasmic actin.