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Related Experiment Videos

Plasmid-mediated beta-lactamase in Branhamella catarrhalis.

C Kamme, I Eliasson, B K Knutson

    Drugs
    |January 1, 1986
    PubMed
    Summary

    Branhamella catarrhalis produces a unique plasmid-mediated beta-lactamase (BRO-1) that differs from other Gram-negative bacteria. This study confirms its genetic transfer and suggests relative substrate affinity index (RSAI) as a screening tool.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Plasmid-mediated beta-lactamases are crucial in bacterial resistance.
    • The BRO-1 beta-lactamase in Branhamella catarrhalis exhibits distinct characteristics.
    • Previous reports suggested variations in beta-lactamases among B. catarrhalis strains.

    Purpose of the Study:

    • To characterize plasmid-mediated beta-lactamases in Branhamella catarrhalis.
    • To investigate potential deviations from the known BRO-1 beta-lactamase.
    • To confirm the conjugational transfer of beta-lactamase production.

    Main Methods:

    • Comparative analysis of beta-lactamase substrate profiles and isoelectric points.
    • Determination of relative substrate affinity index (RSAI).
    • Agarose gel electrophoresis to detect extrachromosomal DNA.

    Main Results:

    • Beta-lactamases from tested B. catarrhalis strains showed no significant deviation from BRO-1.
    • Conjugational transfer of BRO-1 production within B. catarrhalis and from Moraxella nonliquefaciens was confirmed.
    • Four extrachromosomal DNA bands were consistently detected in both beta-lactamase-producing and negative strains.

    Conclusions:

    • The plasmid-mediated beta-lactamase in B. catarrhalis is largely consistent with BRO-1.
    • RSAI is a valuable and efficient tool for screening beta-lactamases in B. catarrhalis.
    • Further geographic studies are recommended to fully understand beta-lactamase diversity.

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