Rheinheimera lutimaris sp. nov., a marine bacterium isolated from coastal sediment

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain YQF-2^T, was isolated from coastal sediment sampled in Jiangsu Province and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 28 °C (range 4-38 °C) and pH 7 (pH 6-10). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YQF-2^T was related to members of the genus Rheinheimera and shared the highest sequence identities with Rheinheimera pacifica KMM 1406^T (98.6%), followed by Rheinheimera aestuarii H29^T (98.4%), Rheinheimera japonica KMM 9513^T (98.3%), Rheinheimera aquimaris SW-353^T (98.3%), Rheinheimera hassiensis E48^T (97.8%) and Rheinheimera muenzenbergensis E49^T (97.7%). The 16S rRNA gene sequence identities between strain YQF-2^T and other members of the genus Rheinheimera were below 97.2%. The digital DNA-DNA hybridization value between strain YQF-2^T and R. pacifica KMM 1406^T was 23.3±2.3%. The average nucleotide identity value between strain YQF-2^T and R. pacifica KMM 1406^T was 79.7%. The unique respiratory quinone was ubiquinone-8. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The strain had summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c), C_16:0, C_12:0 3-OH and iso-C_17:0 3-OH as major fatty acids. The G+C content of the genomic DNA was 50.0 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain YQF-2^T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera lutimaris sp. nov. is proposed, with the type strain YQF-2^T (=KCTC 72184^T=MCCC 1K03663^T).