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Related Experiment Videos

Counting components of the chicken's B cell system.

J R Pink

    Immunological Reviews
    |June 1, 1986
    PubMed
    Summary

    Chicken B lymphocytes originate from precursor cells and undergo rapid gene rearrangement and proliferation within the embryonic bursa. This process generates a vast repertoire of antibody specificities in adult birds.

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    Area of Science:

    • Immunology
    • Developmental Biology
    • Genetics

    Background:

    • Chicken B lymphocytes are crucial for adaptive immunity.
    • Understanding B cell development is key to immune system research.

    Purpose of the Study:

    • To estimate the number of precursor cells and the rate of B cell production in chickens.
    • To characterize the process of immunoglobulin (Ig) gene rearrangement and somatic hypermutation in the avian bursa.

    Main Methods:

    • Order-of-magnitude estimations based on cell numbers and division rates.
    • Analysis of B cell proliferation and Ig gene modification within the embryonic bursa.

    Main Results:

    • B cells originate from <10^5 lymphoid precursors, with rapid Ig gene rearrangement in the bursa.
    • High proliferation rates (10^7-10^8 cells/day) and somatic hypermutation occur antigen-independently.
    • Adult birds possess ~10^10 B cells with at least 10^6 antibody specificities.

    Conclusions:

    • The avian bursa is a highly efficient B cell factory, generating a diverse antibody repertoire.
    • Chicken B cells are self-renewing post-hatching, originating from precursors with pre-arranged or rapidly rearranging Ig genes.

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