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Updated: Oct 10, 2025

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Published on: February 10, 2014
Calcium regulates the interplay between the tight junction and epithelial adherens junction at the plasma membrane
Christopher Mendoza1, Sai Harsha Nagidi2, Kjetil Collett1
1Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA.
Abstract:
The apical junctional complex (AJC) is a membrane protein ultrastructure that regulates cell adhesion and homeostasis. The tight junction (TJ) and the adherens junction (AJ) are substructures of the AJC. The interplay between TJ and AJ membrane proteins to assemble the AJC remains unclear. We employed synthetic biology strategies to express the basic membrane elements of a simple AJC-the adhesive extracellular domains of junctional adhesion molecule A (JAM-A), epithelial cadherin, claudin 1, and occludin-to study their interactions. Our results suggest that calcium concentration fluctuations and JAM-A, acting as an interface molecule between the TJ and AJ, orchestrate their interplay. Calcium affects the secondary structure, oligomerization, and binding affinity of homotypic and heterotypic interactions of TJ and AJ components, thus acting as a molecular switch influencing AJC dynamics.
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