Duodenal microbiota makes an important impact in functional dyspepsia

Area of Science:

• Gastroenterology
• Microbiome Research
• Human Health

Background:

• Functional Dyspepsia (FD) is a common gastrointestinal disorder with multifactorial etiology.
• The role of duodenal microbiota in FD pathogenesis remains incompletely understood.

Purpose of the Study:

• To investigate differences in duodenal mucosal microbiota between FD patients and healthy controls.
• To identify specific microbial taxa and functional pathways associated with FD.

Main Methods:

• Duodenal mucosa and content samples collected via upper gastrointestinal endoscopy from 20 FD patients and 5 healthy subjects.
• Microbiome analysis using 16S rRNA gene sequencing.
• Statistical analysis including LEfSe, FAPROTAX, SPSS, PCoA, and AMOVA.

Main Results:

• Significant differences in microbial diversity (ACE, Shannon, Observed Species indices) and structure (PCoA, AMOVA) between FD patients and controls (P < 0.05).
• Distinct differences in the abundance of specific genera, including Alloprevotella, Peptostreptococcus, Sutterella, and Faecalibacterium.
• Altered duodenal bacterial functions, notably urea decomposition (ureolysis) and fumaric acid respiration, were observed in FD patients (P < 0.05).

Conclusions:

• Duodenal mucosal microbiota composition, diversity, and function are significantly altered in Functional Dyspepsia patients.
• These dysbiotic changes suggest that duodenal microecological disorders may contribute to the development of FD.
• Targeting duodenal microbiota could offer novel therapeutic strategies for Functional Dyspepsia.