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Articles linked to this work by shared authors, journal, and citation graph.

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<i>AcJAZ2L2</i> confers resistance to kiwifruit bacterial canker <i>via</i> regulation of JA signaling and stomatal immunity.

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Corrigendum to "Transcription factor AcNAC056 and AcMADS1 regulate gibberellin 2-oxidase gene AcGA2ox1 induced parthenocarpy in Actinidia eriantha" [Int. J. Biol. Macromol. 322, Part 3 (2025) 146917].

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Transcription factor AcNAC056 and AcMADS1 regulate gibberellin 2-oxidase gene AcGA2ox1 induced parthenocarpy in Actinidia eriantha.

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Effects of plant growth regulator (2,4-D) treatments on fruit development and chemical content in the kiwifruit plant (Actinidia chinensis).

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AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit.

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Related Experiment Video

Updated: Oct 10, 2025

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Comparative Transcriptome Analysis Revealed the Key Genes Regulating Ascorbic Acid Synthesis in Actinidia.

Xiaoying Liu1,2, Xiaodong Xie1, Caihong Zhong1

  • 1CAS Engineering Laboratory for Kiwifruit Industrial Technology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.

International Journal of Molecular Sciences
|December 10, 2021
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Summary

Kiwifruit

Keywords:
GGP3L-galactose pathwayascorbic acidkiwifruittranscriptome analysis

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Area of Science:

  • Plant Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Kiwifruit (Actinidia) is rich in vitamin C (ascorbic acid, AsA), making it a model for studying AsA metabolism.
  • Significant variations in AsA content exist among Actinidia species.

Purpose of the Study:

  • To identify genes regulating AsA synthesis in Actinidia.
  • To elucidate the molecular mechanisms underlying AsA accumulation in kiwifruit.

Main Methods:

  • Transcriptomic analysis of Actinidia species with high and low AsA content.
  • Weighted gene co-expression network analysis and L-galactose pathway integration.
  • Generation and analysis of transgenic kiwifruit overexpressing GGP3.
  • Yeast two-hybrid and BiFC assays to confirm protein interactions.

Main Results:

  • Identified AsA-associated differentially expressed genes (DEGs) linked to abiotic signals and secondary metabolism.
  • GDP-L-galactose phosphorylase-3 (GGP3) was identified as a key candidate gene.
  • Overexpression of GGP3 in transgenic kiwifruit significantly increased AsA content in leaves.
  • Confirmed protein-protein interactions between transcription factors (AcESE3, AcMYBR) and AcGGP3.

Conclusions:

  • Provides insights into the regulatory mechanisms of AsA synthesis in kiwifruit.
  • Identifies GGP3, AcESE3, and AcMYBR as key factors involved in AsA accumulation.
  • Offers potential targets for improving AsA content in kiwifruit through genetic engineering.