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Applications of DNA-Functionalized Proteins.

Zhaoqiu Gong1,2, Yuanyuan Tang1, Ningning Ma1

  • 1College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Jiangsu Key Laboratory of Artificial Functional Materials, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China.

International Journal of Molecular Sciences
|December 10, 2021
PubMed
Summary

DNA nanotechnology enables the creation of novel artificial protein assemblies for diverse applications. This review explores DNA-based protein engineering strategies and their use in catalysis, detection, and biomedicine.

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DNA nanotechnologyDNA–protein hybrid structurecancer diagnosisprecise programming

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Area of Science:

  • Biotechnology and Nanotechnology
  • Structural Biology
  • Biochemistry

Background:

  • Proteins are fundamental to cellular functions and biological processes.
  • Artificial protein assemblies, inspired by nature, are increasingly important.
  • Structural DNA nanotechnology provides powerful tools for engineering protein assemblies.

Purpose of the Study:

  • To review DNA-based protein assemblies and their fabrication.
  • To highlight limitations in the application of these assemblies.
  • To present strategies for overcoming current challenges in various fields.

Main Methods:

  • Leveraging DNA nanotechnology's programmability and addressability.
  • Designing and fabricating novel protein assemblies with precise structures.
  • Reviewing existing literature on DNA-protein assembly applications.

Main Results:

  • Successful fabrication of protein assemblies with unprecedented structures and enhanced functions.
  • Creation of new research fields at the interface of DNA nanotechnology and protein engineering.
  • Identification of key limitations in current applications of DNA-based protein assemblies.

Conclusions:

  • DNA nanotechnology is a transformative approach for creating advanced protein assemblies.
  • Strategies are needed to address limitations in biological catalysis, protein detection, and biomedicine.
  • This field holds significant promise for future scientific and technological advancements.