Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

18
Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
18

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence and burden of coronary artery disease in young adults undergoing clinically indicated coronary CT angiography.

Open heart·2026
Same author

Guangxi Eco-Environmental Health and Aging Study (GEHAS).

BMJ open·2026
Same author

Research opportunities to advance cardiovascular health through a planetary health lens.

American journal of preventive cardiology·2026
Same author

NHANES and the rise of environmental cardiology: Metals, mortality, and the maturation of preventive evidence.

American journal of preventive cardiology·2026
Same author

Interaction Between Air Pollution and Genetic Predisposition to Blood Pressure and LDL-C on Cardiovascular Events.

European journal of preventive cardiology·2026
Same author

Generation of Substantially Metal-Reduced but Aldehyde-Rich Aerosol for E-Cigarette Toxicity Studies.

Journal of visualized experiments : JoVE·2026

Related Experiment Video

Updated: Oct 10, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.7K

Meta-analyses identify DNA methylation associated with kidney function and damage.

Pascal Schlosser1,2, Adrienne Tin3,4, Pamela R Matias-Garcia5,6,7

  • 1Institute of Genetic Epidemiology, Faculty of Medicine and Medical Center - University of Freiburg, Freiburg, Germany. pascal.schlosser@uniklinik-freiburg.de.

Nature Communications
|December 10, 2021
PubMed
Summary
This summary is machine-generated.

Epigenome-wide association studies identified DNA methylation sites linked to kidney function and chronic kidney disease. These findings offer insights into regulatory mechanisms and potential therapeutic targets for kidney disorders.

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

360
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.7K

Related Experiment Videos

Last Updated: Oct 10, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.7K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

360
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

25.7K

Area of Science:

  • Genetics and Epigenetics
  • Nephrology
  • Public Health

Background:

  • Chronic kidney disease (CKD) presents a significant global health challenge.
  • Elevated urinary albumin-to-creatinine ratio (UACR) is a key indicator of kidney damage, crucial for CKD diagnosis and staging.
  • Understanding regulatory mechanisms of kidney function is vital for disease management.

Purpose of the Study:

  • To investigate the epigenetic landscape associated with kidney function and CKD.
  • To identify specific DNA methylation sites (CpGs) related to estimated glomerular filtration rate (eGFR) and UACR.
  • To explore the potential causal roles and biological pathways implicated in kidney disease pathogenesis.

Main Methods:

  • Conducted a blood-based epigenome-wide association study (EWAS) for eGFR (n=33,605) and UACR (n=15,068).
  • Validated associations of specific CpGs in kidney tissue.
  • Utilized enrichment analyses to identify relevant biological pathways.

Main Results:

  • Identified 69 CpGs associated with eGFR and 7 CpGs associated with UACR.
  • Most identified methylation sites showed consistent associations with CKD and albuminuria.
  • CpG associations at JAZF1, PELI1, and CHD2 were validated in kidney tissue; PHRF1, LDB2, CSRNP1, and IRF5 methylation indicated causal effects on kidney function.
  • Enrichment analyses highlighted pathways in hemostasis, blood cell migration (for eGFR), and immune cell activation (for UACR).

Conclusions:

  • Epigenetic variations, specifically DNA methylation, play a role in regulating kidney function and are associated with CKD.
  • Validated findings in kidney tissue and identified potential causal epigenetic factors provide a foundation for further research.
  • The identified pathways offer novel insights into the biological mechanisms underlying kidney disease.