Related Experiment Video
Updated: Jun 19, 2026
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
Published on: February 5, 2018
Presence of Neospora caninum DNA of Wild Birds from Turkey
Muhammet Karakavuk1,2, Hüseyin Can3, Duygu Aldemir4
1Ege University, Ödemiş, Vocational School, İzmir, Turkey
Objective:
Neospora caninum is a protozoon parasite that has a worldwide distribution and mainly causes abortion in cattle and current serological evidence shows that the disease may be also zoonotic. Wild birds play a role as a reservoir of the disease in nature. The study aimed to determine the prensence of N. caninum in wild birds.
Methods:
In this study, the presence of neosporosis in wild birds (n=55) including 22 different species found in the western side of Turkey, was investigated by polymerase chain reaction (PCR). In addition, PCR positive samples were confirmed by sequencing, BLAST, and phylogenetic analysis using MEGA7.
Results:
Obtained results showed that the presence of N. caninum DNA was 5.45% (3/55) in brain-heart homogenates wild birds. The bird species which were found positive for N. caninum were little owl (Athene noctua), common buzzard (Buteo buteo), and little tern (Sternula albifrons). According to phylogenetic analysis and BLAST, all samples were compatible with reference N. caninum isolates.
Conclusion:
To the best of authors' knowledge, this is the first study detecting N. caninum in little tern. In future studies, it may be interesting to investigate the prevalence of N. caninum in other wild animals to elucidate the transmission properties.
