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Related Experiment Video

Updated: Oct 10, 2025

Studying TGF-&#946; Signaling and TGF-&#946;-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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miR-181a Promotes Multiple Protumorigenic Functions by Targeting TGFβR3.

Vida Chitsazzadeh1, Tran N Nguyen2, Alvaro de Mingo Pulido2

  • 1Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

The Journal of Investigative Dermatology
|December 10, 2021
PubMed
Summary
This summary is machine-generated.

MicroRNA-181a (miR-181a) promotes skin cancer (cSCC) development by targeting TGFβR3. Inhibiting miR-181a may offer a new strategy for cSCC chemoprevention.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with a known progression pathway.
  • Identifying molecular targets for chemoprevention is crucial for managing cSCC.
  • Previous research identified miR-181a as a potential oncomiR in cSCC development.

Purpose of the Study:

  • To investigate the role of miR-181a in cSCC pathogenesis.
  • To identify the molecular targets of miR-181a in cSCC.
  • To evaluate miR-181a as a potential target for cSCC chemoprevention.

Main Methods:

  • Analysis of miR-181a and TGFβR3 expression in cSCC.
  • In vitro studies using keratinocytes to assess apoptosis, survival, migration, and invasion.
  • Luciferase reporter assays to confirm direct targeting of TGFβR3 by miR-181a.
  • In vivo studies to assess the effect of miR-181a inhibition on tumor growth.

Main Results:

  • miR-181a is upregulated and TGFβR3 is downregulated in cSCC.
  • miR-181a overexpression (OE) and TGFβR3 knockdown (KD) promote protumorigenic properties, including suppressed apoptosis and enhanced survival, migration, and invasion.
  • miR-181a directly targets TGFβR3, influencing TGFβ signaling pathways (SMAD3, SNAIL, SLUG).
  • Inhibition of miR-181a in vivo compromises tumor growth.

Conclusions:

  • miR-181a acts as an oncomiR in cSCC by targeting TGFβR3.
  • The miR-181a/TGFβR3 axis plays a significant role in cSCC development.
  • Targeting miR-181a represents a promising therapeutic strategy for cSCC chemoprevention.