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Cell sodium activity and sodium pump function in frog skin.

J F García-Díaz, G Klemperer, L M Baxendale

    The Journal of Membrane Biology
    |January 1, 1986
    PubMed
    Summary
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    Cellular sodium (Na) activity in frog skin is crucial for regulating sodium transport. This study reveals how apical sodium entry influences intracellular sodium levels and basolateral active sodium flux.

    Area of Science:

    • Physiology
    • Cell Biology
    • Membrane Transport

    Background:

    • Cellular sodium (Na) activity (acNa) is a key determinant of epithelial sodium transport.
    • Understanding the relationship between apical Na entry and intracellular Na handling is essential for comprehending kidney and skin physiology.

    Purpose of the Study:

    • To measure intracellular Na activity (acNa) in frog skin under varying apical Na conditions.
    • To elucidate the dynamic relationship between apical Na entry, acNa, and basolateral active Na flux.

    Main Methods:

    • Utilized microelectrode impalement techniques in short-circuited frog skin preparations.
    • Manipulated apical and serosal bathing solutions to control Na concentrations and paracellular conductance.
    • Measured acNa and cell current (Ic) simultaneously.

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    Main Results:

    • Control acNa was 8 ± 2 mM, decreasing to 3 mM with amiloride or apical Na removal.
    • Decreased apical [Na] led to parallel reductions in acNa and Ic.
    • Upon Na restoration, Ic recovered rapidly, while acNa increased exponentially (time constant 1.8 ± 0.7 min).
    • Pump Na flux correlated linearly with acNa (2-12 mM range).

    Conclusions:

    • Intracellular Na activity (acNa) is a critical factor linking apical Na entry to basolateral active Na transport in frog skin.
    • The Na transport system exhibits a dynamic response to changes in apical Na availability.
    • These findings provide insights into the regulatory mechanisms of sodium homeostasis in epithelial tissues.