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Probing allosteric interactions in homo-oligomeric molecular machines using solution NMR spectroscopy

Yuki Toyama1,2,3, Lewis E Kay4,2,3,5

  • 1Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.

Proceedings of the National Academy of Sciences of the United States of America
|December 11, 2021
PubMed
Summary

Solution Nuclear Magnetic Resonance (NMR) spectroscopy reveals molecular machine dynamics. This study demonstrates directly observing intersubunit allosteric communication in large protein systems using NMR, enhancing our understanding of cellular homeostasis.

Keywords:
ClpP1P2human high-temperature requirement A2 proteinmethyl-TROSYp97/VCPproteasome

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