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Immunoenhancement and antioxidative damage effects of Polygonum Cillinerve polysaccharide on RAW264.7 cells
Yizhou Lv1, Zhenxiu Liu1, Xueqin Duan1
1College of Veterinary Medicine, Northwest A&F University, Yangling District, Xianyang, Shaanxi, P.R. China.
Objectives:
The effects of Polygonum Cillinerve polysaccharide (PCP) on the immune and antioxidant activity were studied.
Methods:
The effects of PCP on cell proliferation, phagocytic activity, cell uptake, the secretion of NO, iNOS, IL-6, IL-12, CAT and POD, intracellular ROS, cell apoptosis and antioxidative mechanism were measured by MTT, ELISA, fluorescence staining, flow cytometry and western blot.
Key Findings:
The results showed that PCP had no toxic effect at 31.25-1.95 µg/ml, could improve the uptake of neutral red and fluorescein isothiocyanate-labelled ovalbumin and promote the release of nitric oxide and nitric oxide synthase. Moreover, PCP also could promote the secretion of IL-6 and IL-12. The damage of RAW264.7 cells induced by hydrogen peroxide was significantly alleviated by PCP at 15.63-0.975 µg/ml. The mechanism of antioxidative damage might be that PCP inhibited the upstream p38 and the phosphorylation of JNK and ERK proteins, and down-regulated caspase 3 and up-regulated the protein expressions of cytochrome C and Bcl-2, finally PCP improved the antioxidative capacity and protected the oxidative damage of cells.
Conclusions:
These results indicated that PCP had the better immunopotentiation and antioxidative damage activity.
