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Tumor microenvironment based stimuli-responsive CRISPR/Cas delivery systems: A viable platform for interventional

Nanyang Tang1, Qian Ning2, Zewei Wang1

  • 1Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, and Institute of Pharmacy & Pharmacology, School of Pharmaceutical Science, University of South China, Hengyang 421001, China; Hunan Province Key Laboratory for Antibody-Based Drug and Intelligent Delivery System, School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.

Colloids and Surfaces. B, Biointerfaces
|December 11, 2021
PubMed

Insights

Stimuli-responsive delivery systems enhance clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) gene therapy for cancer. These systems improve tumor-specific editing and reduce genotoxicity for better therapeutic outcomes.

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Oncology

Background:

  • Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems offer versatile cancer gene therapy potential.
  • Challenges include non-specific delivery and genotoxicity, limiting clinical applications.
  • The tumor microenvironment (TME) presents opportunities for targeted delivery strategies.

Purpose of the Study:

  • To review CRISPR/Cas systems used in cancer therapy.
  • To discuss stimuli-responsive delivery systems for TME-specific CRISPR/Cas applications.
  • To summarize the efficiency of these advanced delivery systems.

Main Methods:

  • Review of current literature on CRISPR/Cas systems in cancer gene therapy.
  • Analysis of various stimuli-responsive materials and their integration into CRISPR/Cas carriers.
  • Evaluation of in vitro and in vivo studies on CRISPR/Cas-loaded carriers.

Main Results:

  • CRISPR/Cas systems are adaptable tools for cancer gene therapy.
  • Stimuli-responsive delivery systems enable tumor-specific genome editing.
  • Dual- and multiple-responsive carriers demonstrate enhanced spatiotemporal control.
  • CRISPR/Cas-loaded carriers show promising editing efficiencies in preclinical models.

Conclusions:

  • Stimuli-responsive CRISPR/Cas delivery systems are a promising strategy for cancer gene therapy.
  • These systems can overcome limitations of non-specific delivery and genotoxicity.
  • Further development holds potential for improved cancer treatment outcomes.
Keywords:
CRISPR/CasGene therapyStimuli-responsive deliveryTumor microenvironment

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