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Related Experiment Videos

Prolactin binding protein in human milk.

M T Clandinin, J E Chappell, D Mager

    Endocrinologia Experimentalis
    |August 1, 1986
    PubMed
    Summary

    Human prolactin binding in milk differs between mothers of preterm and full-term infants. A soluble protein fraction in milk selectively binds human prolactin, with variations observed in binding capacity.

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    Area of Science:

    • Human milk composition
    • Lactation biology
    • Neonatal nutrition

    Background:

    • Human prolactin plays a crucial role in lactation and infant development.
    • Milk composition can vary based on gestational age at birth.
    • Understanding prolactin's role in early lactation is vital for infant health.

    Purpose of the Study:

    • To investigate the presence and characteristics of a prolactin binding protein in human milk.
    • To compare prolactin binding in milk from mothers of preterm infants versus full-term infants.
    • To explore factors influencing prolactin binding in early lactation.

    Main Methods:

    • Human milk samples collected at day 16 postpartum from mothers of preterm (28-30 weeks gestation) and full-term infants.
    • 125I-labelled human prolactin used in a binding assay.
    • Sephadex G-100 column chromatography to isolate soluble protein fractions.

    Main Results:

    • A soluble protein fraction capable of binding human prolactin was identified in 7 out of 12 milk samples.
    • Prolactin binding was detected in milk from both preterm and full-term infant groups.
    • Binding was not apparent in 5 samples, suggesting variability in the presence or activity of the binding protein.

    Conclusions:

    • A specific prolactin binding protein exists in early human milk.
    • Variability in prolactin binding suggests potential roles for maternal prolactin levels, binding capacity, and free prolactin concentrations.
    • Further research is needed to elucidate the functional significance of this milk protein-prolactin interaction for infant development.

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