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Prolactin binding protein in human milk
Milk samples at day 16 of lactation were from six mothers delivering infants at 28-30 weeks of gestation and six mothers delivering normal full term infants. These early morning samples were used in a prolactin binding assay to determine whether 125I-labelled human prolactin was selectively bound to a soluble protein fraction. After Sephadex G-100 column chromatography, 7 samples contained a protein fraction which bound human prolactin. Binding was not apparent in the remaining 5 samples. Prior binding of maternal prolactin, total binding capacity and differing free prolactin levels may be significant factors responsible for these observations.
Milk samples at day 16 of lactation were from six mothers delivering infants at 28-30 weeks of gestation and six mothers delivering normal full term infants. These early morning samples were used in a prolactin binding assay to determine whether 125I-labelled human prolactin was selectively bound to a soluble protein fraction. After Sephadex G-100 column chromatography, 7 samples contained a protein fraction which bound human prolactin. Binding was not apparent in the remaining 5 samples. Prior binding of maternal prolactin, total binding capacity and differing free prolactin levels may be significant factors responsible for these observations.