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Experimental Column Setup for Studying Anaerobic Biogeochemical Interactions Between Iron OxyHydroxides, Trace Elements, and Bacteria
Published on: December 19, 2017
Performances and mechanisms of microbial nitrate removal coupling sediment-based biochar and nanoscale zero-valent
Xiaohui Liu1, Jia Wei1, Yaodong Wu1
1College of Architecture Engineering, Beijing University of Technology, 100 Pingleyuan, Chaoyang District, Beijing 100124, China.
Abstract:
Immobilized microorganism technology has attracted increasing attention for high concentration of microbes, low cell loss and high resistance to impact of environment. The microbial reduction of nitrate in the presence of sediment-based biochar (SBC) and nanoscale zero-valent iron (nZVI) was investigated in four different free systems. NZVI-SBC/bacteria system realized the best nitrate removal of 97.61% within 3 days through the synergistic effect of SBC and nZVI on denitrifying bacteria. Accumulation of nitrite and ammonium in nZVI-SBC/bacteria system also decreased. High-throughput sequencing results showed that the proportion of denitrifying bacteria in microbial community structure increased after adding nZVI-SBC. The performance of nitrate removal was then studied through PVA/SA-immobilization. Immobilized active pellets performed better nitrate removal (98.89%) and stronger tolerance under different conditions than the free bacterial cells. Overall, this study provided a promising approach by utilizing SBC and nZVI for the bio-remediation of nitrate-contaminated water in practical application.
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