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Updated: Oct 10, 2025
Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
Published on: November 4, 2021
[Effects of miR-34b/c and miR-449 on the morphology and function of efferent ductules in mice: A
Yu-Jie Wu1, Yue Liu1, Yan-Qin Hu1
1Shanghai Key Laboratory of Reproductive Medicine / Department of Histology, Embryology, Genetics and Developmental Biology, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China .
Objective:
To investigate the role of miR-34b/c and miR-449 in maintaining the normal structure and function of efferent ductules and explore the molecular mechanism of infertility in miR-34b/c－/－ and miR-449－/－ dKO mice.
Methods:
We observed the morphology of mouse efferent ductules by HE staining and analyzed the gene expressions in the efferent ductules of the wild-type and miR-34b/c－/－ and miR-449－/－ dKO mice by RNA sequencing. Then we screened the possible target genes of these two miRNA clusters and analyzed them along with the differentially expressed genes, followed by verification of the sequencing results by qRT-PCR.
Results:
Compared with the wild-type, the dKO mice showed morphologically abnormal efferent ductules and significantly decreased expressions of the genes involved in the formation of cilia and related to the transportation of water, ion and protein in the efferent ductules.
Conclusions:
The deletion of miR-34b/c and miR-449 led to morphological abnormality of efferent ductules and dysfunction of aberrant cilia motility and reabsorption in the efferent ductules of dKO mice, resulting in infertility.
