Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-activity studies of interleukin-2.

F E Cohen, P A Kosen, I D Kuntz

    Science (New York, N.Y.)
    |October 17, 1986
    PubMed
    Summary

    Researchers used computational methods to predict the structure of interleukin-2 (IL-2), a key immune protein. The most likely structure is a four-helix bundle, consistent with experimental data and identifying a potential receptor binding site.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A CONTRIBUTION TO THE UNDERSTANDING OF THE PRIMARY QUANTUM CONVERSION IN PHOTOSYNTHESIS. UCRL-11212.

    UCRL [reports]. U.S. Atomic Energy Commission·2014
    Same author

    Viral chemokine receptors and chemokines in human cytomegalovirus trafficking and interaction with the immune system. CMV chemokine receptors.

    Current topics in microbiology and immunology·2002
    Same author

    A genetic algorithm for structure-based de novo design.

    Journal of computer-aided molecular design·2002
    Same author

    Engineering the prion protein using chemical synthesis.

    The journal of peptide research : official journal of the American Peptide Society·2002
    Same author

    Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein.

    Journal of molecular biology·2001
    Same author

    The impact of whole genome sequence data on drug discovery--a malaria case study.

    Molecular medicine (Cambridge, Mass.)·2001

    Area of Science:

    • Immunology
    • Structural Biology
    • Computational Biology

    Background:

    • Interleukin-2 (IL-2) plays a critical role in immune responses.
    • Understanding IL-2's structure is essential for elucidating its biological activity.

    Purpose of the Study:

    • To predict the secondary and tertiary structure of IL-2 using computational methods.
    • To identify potential functional regions of IL-2, such as its receptor binding site.

    Main Methods:

    • Application of computer-assisted predictive methods for protein secondary structure assignment.
    • Prediction of tertiary protein structure from primary sequence and secondary structure data.
    • Analysis of predicted structures for compatibility with existing experimental data (disulfide bridges, antibody binding).

    Main Results:

    • Four topological families of protein structures were generated for IL-2.
    • The most plausible predicted structure is a right-handed fourfold alpha-helical bundle.
    • This model aligns with experimental data, including circular dichroism and site-directed mutagenesis.
    • A specific region (residues Leu36-Met46) was identified as potentially crucial for IL-2 receptor binding.

    Conclusions:

    • The predicted alpha-helical bundle structure provides a plausible model for IL-2.
    • The identified receptor binding region offers insights into IL-2's mechanism of action.
    • Computational structure prediction is a valuable tool for understanding protein function.

    Related Experiment Videos