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Updated: Oct 10, 2025
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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
Published on: November 19, 2019
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Long noncoding RNA SNHG20 promotes prostate cancer progression via upregulating DDX17
Xing-Cheng Wu1, Wei-Gang Yan1, Zhi-Gang Ji1
1Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Peking, China.
Archives of Medical Science : AMS
|December 13, 2021
Summary
Long noncoding RNA SNHG20 promotes prostate cancer (PCa) cell growth and spread. SNHG20 acts as a competing endogenous RNA (ceRNA) to increase DDX17 levels, suggesting SNHG20 as a potential therapeutic target for PCa.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) play critical roles in cancer development.
- LncRNA SNHG20 is implicated in various cancers, but its role in prostate cancer (PCa) remains unclear.
Purpose of the Study:
- To investigate the clinical significance, biological functions, and molecular mechanisms of lncRNA SNHG20 in prostate cancer.
- To explore the potential of SNHG20 as a therapeutic target for PCa.
Main Methods:
- Quantitative real-time PCR (RT-qPCR) to detect SNHG20 expression in PCa tissues and cell lines.
- Cell proliferation and migration assays (CCK-8, EdU, Transwell) to assess SNHG20's functional roles.
- Dual luciferase reporter assay, RT-qPCR, and Western blot to elucidate the regulatory mechanism involving DDX17.
Main Results:
- SNHG20 is highly expressed in PCa and correlates with advanced Gleason score and tumor stage.
- Overexpression of SNHG20 enhances PCa cell proliferation and migration, while knockdown inhibits these processes.
- SNHG20 functions as a competing endogenous RNA (ceRNA) to upregulate DDX17, which also exhibits oncogenic roles in PCa.
Conclusions:
- SNHG20 promotes PCa cell proliferation and migration by upregulating DDX17 via a ceRNA mechanism.
- SNHG20 represents a potential novel therapeutic target for prostate cancer treatment.
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