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Updated: Oct 10, 2025
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Mir-204 Regulates LPS-Induced A549 Cell Damage by Targeting FOXK2
Shufen Li1, Lifen Zhao2,3, Xujiong Li1
1Department of Physiology, Changzhi Medical College, Changzhi, Shanxi 046000, China.
Objective:
To assess whether miR-204 and HA affect A549 cell injury induced by lipopolysaccharide. Material and Methods. A549 cells were treated with hirsutanol A, and cell damage was induced by LPS followed by analysis of cell proliferation by CCK-8, cell apoptosis by flow cytometry, apoptosis-related protein expression by western blot, downstream target of miR-20 by dual-luciferase reporter gene, and inflammatory factors by ELISA and PCR.
Results:
LPS can significantly inhibit the viability of A549 cells, induce cell apoptosis, and promote the release of IL-6, IL-1β, and TNF-α, while HA pretreatment can target FOXK2 by upregulating miR-204 levels, thereby alleviating apoptosis and promoting cell viability and at the same time inhibiting the release of inflammatory factors by inhibiting the activation of NF-κB.
Conclusions:
miR-204 participates in the protection of HA acute lung injury by targeting FOXK2.
