Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

14.2K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.2K
Liver Regeneration01:24

Liver Regeneration

3.5K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The interaction of Gα13 with integrin β1 mediates cell migration by dynamic regulation of RhoA.

Molecular biology of the cell·2015
Same author

Overcoming difficulty in diagnosis and differential diagnosis of Crohn's disease: the potential role of serological and genetic tests.

Expert review of molecular diagnostics·2015
Same author

The presence of primary sclerosing cholangitis in patients with ileal pouch anal- anastomosis is associated with an additional risk for vitamin D deficiency.

Gastroenterology report·2015
Same author

Perioperative Blood Transfusion and Postoperative Outcome in Patients with Crohn's Disease Undergoing Primary Ileocolonic Resection in the "Biological Era".

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2015
Same author

Postoperative Fluid Overload is a Useful Predictor of the Short-Term Outcome of Renal Replacement Therapy for Acute Kidney Injury After Cardiac Surgery.

Medicine·2015
Same author

Intranuclear biophotonics by smart design of nuclear-targeting photo-/radio-sensitizers co-loaded upconversion nanoparticles.

Biomaterials·2015

Related Experiment Video

Updated: Oct 10, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.8K

Dissecting the single-cell transcriptomeunderlying chronic liver injury.

Junjun Wang1,2, Wei Hu1, Zhenyang Shen1,2

  • 1Department of Gastroenterology, Precision Research Center for Refractory Diseases, Institute for Clinical Research, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.

Molecular Therapy. Nucleic Acids
|December 13, 2021
PubMed
Summary

This study used single-cell RNA sequencing to map chronic liver injury in mice, identifying key cell types and gene markers like Ets1. Findings reveal altered cell communication, offering insights for treating chronic liver diseases.

Keywords:
cell-cell communicationschronic liver injurymacrophagesingle-cell transcriptome

More Related Videos

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

6.3K
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
08:52

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

11.3K

Related Experiment Videos

Last Updated: Oct 10, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.8K
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

6.3K
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
08:52

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

11.3K

Area of Science:

  • Hepatology
  • Immunology
  • Genomics

Background:

  • Chronic liver disease (CLD) is a global health issue with limited effective treatments.
  • A detailed understanding of chronic liver injury at the single-cell level is currently lacking.

Purpose of the Study:

  • To systematically characterize chronic liver injury using single-cell RNA sequencing.
  • To identify novel molecular markers and cellular communication patterns in liver injury models.

Main Methods:

  • Established choline-deficient, ethionine-supplemented (CDE) and 3,5-diethoxycarbonyl 1,4-dihydrocollidinen (DDC) mouse models for chronic liver injury.
  • Performed single-cell RNA sequencing (scRNA-seq) on 16,389 high-quality cells.
  • Utilized transcriptional trajectory, differential gene expression, and cell-cell communication analyses.

Main Results:

  • Identified 12 major cell types, with macrophages and endothelial cells being predominant.
  • Revealed distinct cellular expression patterns between CDE and DDC models.
  • Discovered potential liver injury markers (Ets1, Gda, Itgam, Sparc) and identified numerous differentially expressed genes in macrophages, including 413 exclusively expressed genes involved in immune processes.
  • Observed significant reduction in cell-cell interactions, particularly in the DDC model.

Conclusions:

  • The study provides a comprehensive single-cell transcriptional landscape of chronic liver injury.
  • Findings enhance understanding of molecular mechanisms underlying liver disease progression.
  • Identified potential targets for clinical intervention in chronic liver diseases.