Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glomerular Filtration01:15

Glomerular Filtration

2.5K
The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
2.5K
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

3.6K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
3.6K
Renal Drug Excretion: Glomerular Filtration01:02

Renal Drug Excretion: Glomerular Filtration

644
The kidney serves as the primary organ responsible for eliminating drugs and their metabolites from the body. This process, known as renal elimination, starts with glomerular filtration and results in urine formation. Each kidney houses millions of functional units called nephrons, where urine production occurs. A nephron has two main components: a renal corpuscle and a renal tubule.
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles....
644
Glomerular Filtration: Net Filtration Pressure01:26

Glomerular Filtration: Net Filtration Pressure

4.3K
Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP).
GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal...
4.3K
Renal Corpuscle01:20

Renal Corpuscle

3.7K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
3.7K
Physiology of the Genitourinary System I: Renal Blood Flow and Glomerular Filtration01:29

Physiology of the Genitourinary System I: Renal Blood Flow and Glomerular Filtration

153
The kidneys are vital organs responsible for regulating blood filtration, waste excretion, and fluid balance, all of which are crucial for maintaining homeostasis. Renal physiology examines renal blood flow, glomerular filtration, and urine formation, ensuring the body’s internal environment remains stable.Renal Blood FlowThe kidneys receive about 20-25% of the cardiac output, typically around 1200 mL of blood per minute in an average adult. Blood flows into the kidneys through the renal...
153

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan.

Cell metabolism·2026
Same author

Extracellular vesicle miR-93-5p cargo regulates glomerular endothelial cell damage in Alport syndrome.

JCI insight·2026
Same author

Hypothyroidism impairs skeletal muscle regeneration after injury by altering myogenic and nonmyogenic pathways.

JCI insight·2026
Same author

Response to the Letter to the Editor Entitled "Revisiting Vincristine for FSGS: Proof-of-Mechanism Arrives, but Whom Should we Treat?"

Kidney international reports·2026
Same author

Endothelial cell-released CD93 contributes to podocyte injury in idiopathic nephrotic syndrome.

Science translational medicine·2026
Same author

Editorial: Stem cells and kidney regeneration: transforming renal medicine.

Frontiers in bioengineering and biotechnology·2025

Related Experiment Video

Updated: Oct 10, 2025

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.6K

Emerging Technologies to Study the Glomerular Filtration Barrier.

Emma Gong1, Laura Perin1,2, Stefano Da Sacco1,2

  • 1Division of Urology, GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics, Children's Hospital Los Angeles, Saban Research Institute, Los Angeles, CA, United States.

Frontiers in Medicine
|December 13, 2021
PubMed
Summary

New technologies like single cell RNA sequencing and kidney organoids are improving our understanding of kidney disease and proteinuria. This knowledge aids in developing personalized treatments for proteinuric diseases.

Keywords:
Proteinuriaglomerular filtration barrierkidney diseasekidney organoidskidney-on-a-chipsingle cell transcriptomicsspatial transcriptomics

More Related Videos

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

7.1K
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

13.9K

Related Experiment Videos

Last Updated: Oct 10, 2025

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
11:13

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Published on: April 17, 2013

10.6K
Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

7.1K
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
10:14

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

13.9K

Area of Science:

  • Nephrology
  • Genomics
  • Bioinformatics

Background:

  • Kidney disease involves glomerular dysfunction and proteinuria.
  • The glomerular filtration barrier's complexity hinders understanding cellular and molecular changes.
  • Podocytes, endothelial cells, and basement membrane form the filtration barrier.

Purpose of the Study:

  • To review advancements in understanding glomerular pathophysiology.
  • To highlight the role of novel technologies in studying kidney disease.
  • To explore the development of personalized therapies for proteinuric conditions.

Main Methods:

  • Single cell RNA sequencing.
  • Bioinformatics-based spatial transcriptomics.
  • In vitro kidney organoids and glomerulus-on-a-chip models.

Main Results:

  • These technologies offer unprecedented insights into glomerular cell function and interactions.
  • Advanced techniques reveal molecular drivers of proteinuria.
  • Data facilitates a deeper comprehension of kidney disease mechanisms.

Conclusions:

  • Novel technologies are crucial for dissecting glomerular pathophysiology.
  • Understanding cellular and molecular changes aids personalized medicine.
  • This research paves the way for targeted therapies for proteinuric diseases.