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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Published on: May 2, 2018
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16S rRNA gene amplicon sequence data from sunflower endosphere bacterial community
Olubukola Oluranti Babalola1, Bartholomew Saanu Adeleke1, Ayansina Segun Ayangbenro1
1Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
Data in Brief
|December 13, 2021
Summary
This study explored the endophytic bacterial communities in sunflower plants from South Africa, revealing their diversity and potential for biofertilizer development to promote sustainable agriculture.
Area of Science:
- Microbiology
- Plant Science
- Bioinformatics
Background:
- Plant endophytes play crucial roles in plant health and nutrient cycling.
- Understanding endophytic bacterial communities is vital for developing sustainable agricultural practices and biofertilizers.
Purpose of the Study:
- To characterize the endophytic bacterial diversity in sunflower roots and stems.
- To explore the potential of these bacteria as bioincentives for biofertilizer formulation.
- To investigate the influence of soil type (organic vs. non-organic) and plant growth stage on bacterial communities.
Main Methods:
- 16S rRNA gene amplicon sequencing was used to profile bacterial communities.
- Genomic DNA was extracted using commercial kits and amplified via polymerase chain reaction.
- Sequence data was processed using QIIME on the Nephele microbial bioinformatics platform.
- Sequences were deposited in the NCBI Sequence Read Archive (SRA).
Main Results:
- The study revealed the structure and functions of endophytic bacterial communities in sunflower.
- Data provides insights into bacterial diversity in response to different soil types and plant developmental stages.
- The findings lay the groundwork for identifying beneficial microbes for biofertilizer applications.
Conclusions:
- The endophytic bacterial communities in sunflower exhibit diversity that can be leveraged for agricultural benefits.
- This research contributes to the exploration of bioincentives for sustainable agriculture through biofertilizer development.
