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Related Experiment Video

Updated: Oct 10, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

circATP2A2 promotes osteosarcoma progression by upregulating MYH9.

Xin Cao¹, Xianfeng Meng¹, Peng Fu¹

¹Department of Trauma and Orthopaedics, Shengli Oilfield Central Hospital, Dongying, Shandong, China.

Open Medicine (Warsaw, Poland)

|December 13, 2021

Summary

Circular RNA hsa_circ_0028173 (circATP2A2) promotes osteosarcoma (OS) progression by upregulating MYH9 via sponging miR-335-5p. CircATP2A2 may serve as a diagnostic and prognostic biomarker for OS treatment.

Keywords:

MYH9 OS circATP2A2 miR-335-5p

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Last Updated: Oct 10, 2025

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Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Osteosarcoma (OS) is a highly metastatic primary bone cancer.
CircRNA hsa_circ_0028173 (circATP2A2) has been implicated in OS progression, but its specific role requires validation.

Purpose of the Study:

To elucidate the biological role and underlying mechanism of circATP2A2 in osteosarcoma.
To investigate circATP2A2 as a potential diagnostic and prognostic biomarker for OS.

Main Methods:

Quantitative real-time PCR (qRT-PCR) to measure circATP2A2, MYH9, and miR-335-5p expression.
Cell proliferation, glycolysis, migration, and invasion assays in vitro.
In vivo experiments to assess tumor growth and metastasis.

Main Results:

CircATP2A2 and MYH9 were upregulated, while miR-335-5p was downregulated in OS tissues and cells.
High circATP2A2 expression correlated with shorter overall survival in OS patients (AUC=0.77).
CircATP2A2 knockdown inhibited OS cell proliferation, glycolysis, migration, and invasion, both in vitro and in vivo.

Conclusions:

CircATP2A2 promotes osteosarcoma malignancy and glycolysis by upregulating MYH9 through sponging miR-335-5p.
CircATP2A2 serves as a potential diagnostic and prognostic biomarker for osteosarcoma.
Targeting the circATP2A2/miR-335-5p/MYH9 axis offers a promising therapeutic strategy for osteosarcoma.