Formation of Visible Aggregates between Rolling Circle Amplification Products and Magnetic Nanoparticles as a
Darío Sánchez Martín1, Reinier Oropesa-Nuñez2, Teresa Zardán Gómez de la Torre1
1Department of Material Sciences and Engineering, Division of Nanotechnology and Functional Materials, Ångström Laboratory, Uppsala University, 751 21 Uppsala, Sweden.
Abstract:
Visual detection of rolling circle amplification products (RCPs) has been achieved by specific aggregation with magnetic nanoparticles. The method presented here reliably generates aggregates in 1.5 h; these are visible to the naked eye in samples containing at least 0.4 fmol of RCPs. In addition, alternate current susceptometry and absorbance spectroscopy have also been used to quantify the amplified products. The specificity of the detection method was tested, and no non-specific aggregation was detected in samples containing up to 20 fmol of non-complementary amplified DNA. This method is a versatile tool for detecting pathogenic DNA in point-of-care diagnostics, with no readout equipment required. However, chips and automated assays can be used in conjugation with the developed method since detection and quantification can be achieved by commercially available readout instruments.
