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Updated: Oct 10, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry
Sanghwan Park1, Chang Young Lee2
1School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea.
Abstract:
Mass spectrometry imaging (MSI) allows label-free detection of a wide range of biomolecules and simultaneously provides their spatial distributions. In particular, MSI by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) has been widely used in biomolecule analysis. However, quantitation in MALDI-MSI is limited by matrix-deposition heterogeneity, analyte extraction area, and analyte-matrix cocrystallization. In this chapter, a microstructured PDMS stamp is utilized to precisely control the matrix deposition area and the analyte extraction area. We describe here simple steps-including stamp fabrication, matrix application, and data-acquisition guideline-for the quantitative analysis of adsorbed peptides on hydrophobic surfaces.
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