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Updated: Oct 10, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Quantitative Mass Spectrometry Imaging with Liquid Microjunction Surface Sampling.

Qian Wu1, Hongmei Lu2

  • 1College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, P. R. China.

Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2021
PubMed
Summary
This summary is machine-generated.

Quantitative mass spectrometry imaging (MSI) is now feasible using liquid microjunction surface sampling (LMJSS). This method enables absolute quantitation of lipids and metabolites in tissue samples.

Keywords:
Calibration methodsLipidomicsLiquid microjunction surface samplingMetabolomicsQuantitative mass spectrometry imaging

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Area of Science:

  • Analytical Chemistry
  • Biomedical Imaging
  • Metabolomics

Background:

  • Mass spectrometry imaging (MSI) offers valuable chemical spatial distribution data but struggles with absolute quantitation.
  • Ambient liquid extraction-based MSI techniques, like liquid microjunction surface sampling (LMJSS), show promise for improved quantitation.
  • Direct doping of standards in the extraction solvent is a key advantage of LMJSS for quantitative analysis.

Purpose of the Study:

  • To present detailed experimental protocols for quantitative MSI using LMJSS.
  • To outline data processing methods for achieving absolute quantitation with LMJSS.
  • To demonstrate the application of LMJSS for the quantitative analysis of endogenous compounds in tissue samples.

Main Methods:

  • Development and description of experimental protocols for quantitative MSI.
  • Implementation of liquid microjunction surface sampling (LMJSS) for direct analyte extraction.
  • Utilizing internal standards doped directly into the extraction solvent for calibration.

Main Results:

  • Established detailed protocols for quantitative MSI with LMJSS.
  • Successfully applied LMJSS for the absolute quantitation of endogenous lipids.
  • Demonstrated absolute quantitation of small metabolites in tissue samples using LMJSS.

Conclusions:

  • Quantitative MSI using LMJSS is a viable and effective approach.
  • The described methods enable absolute quantitation of diverse biomolecules in complex matrices.
  • LMJSS represents a significant advancement for chemical analysis in biological tissues.