Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural Basis of pppGpp Binding to the N-Terminal Domain of the Bifunctional RelA/SpoT Homolog Rel<sub>Seq</sub>: Crystal Structure and MD Analysis.

International journal of molecular sciences·2026

Same author

A Microscale Platform for the Comprehensive Analysis of Bacterial Translation Initiation.

International journal of molecular sciences·2026

Same author

PrfH accelerates repair of colicin E3 - damaged translating ribosomes by RtcB2.

RNA biology·2026

Same author

Plant Innate Immunity: Crosstalk of Signaling Pathways.

Biochemistry. Biokhimiia·2026

Same author

Red clover RNA virus 1: functional analysis of ORF2 protein, associations with co-infecting viruses, and seed transmission.

Archives of virology·2026

Same author

Plant virus seed transmission: AGO5-mediated defense and viral counter-defense.

Frontiers in plant science·2026

Search research articles

Related Experiment Video

Updated: Oct 10, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

RNA Binding by Plant Serpins in vitro.

Eugene A Tolstyko^1,2, Denis A Chergintsev³, Olga A Tolicheva²

¹Department of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.

Biochemistry. Biokhimiia

|December 14, 2021

Summary

Plant serpins, like Arabidopsis thaliana AtSerpin1, bind to transfer RNA (tRNA). These serpins recognize unique structural motifs in RNA, indicating a role beyond protease inhibition.

Keywords:

RNA binding RNA structure RNA-binding protein microRNA phloem serpin tRNA

More Related Videos

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Related Experiment Videos

Last Updated: Oct 10, 2025

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Assaying Proteasomal Degradation in a Cell-free System in Plants

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

Molecular Biology
Plant Biochemistry

Background:

Serpins are protease inhibitors found across all life forms.
Most plant serpins lack functional characterization, unlike Arabidopsis thaliana AtSerpin1 and Cucurbita maxima CmPS1.
CmPS1 is a phloem protein known to bind RNA, particularly tRNA.

Purpose of the Study:

To investigate the RNA-binding capabilities of plant serpins.
To determine the specificity of interactions between plant serpins and RNA.
To elucidate the structural basis for plant serpin-RNA complex formation.

Main Methods:

Analysis of tRNA species bound by AtSerpin1 and CmPS1.
Testing the binding of CmPS1 to microRNA precursors (pre-miR390) and their mutants.
Structural analysis of plant serpin-RNA interactions.

Main Results:

Both AtSerpin1 and CmPS1 form complexes with tRNA.
Neither serpin exhibited strict selectivity for individual tRNA species.
CmPS1 binding to pre-miR390 depends on the presence of unpaired nucleotide residues in the hairpin stem.

Conclusions:

Plant serpins interact with structured RNA, not specific RNA sequences.
The interaction involves recognition of unique spatial motifs formed by unpaired nucleotides in RNA duplexes.
Plant serpins may have roles involving structured RNA recognition beyond protease inhibition.