Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Metabolism of Chemolithotrophs

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Environmental selection constrains metabolic network architecture despite taxonomic turnover in anaerobic digestion communities.

The ISME journal·2026

Same author

Towards sustainable serum-free media development from alternative sources for cultivated meat.

Food research international (Ottawa, Ont.)·2026

Same author

Metabolic feedbacks drive population dynamics and can lead to oscillations among leaf bacteria.

Nature communications·2026

Same author

Pan-cancer analysis in the real-world setting uncovers immunogenomic drivers of acquired resistance post-immunotherapy.

Journal for immunotherapy of cancer·2026

Same author

A community reconstruction of Chinese hamster metabolism and structural systems biology elucidate metabolic rewiring in lactate-free CHO cells.

Cell systems·2026

Same author

Genome-scale metabolic model-guided systematic framework for designing customized live biotherapeutic products.

NPJ systems biology and applications·2025

Search research articles

Related Experiment Video

Updated: Oct 10, 2025

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

Enhancing Microbiome Research through Genome-Scale Metabolic Modeling.

Nana Y D Ankrah¹, David B Bernstein², Matthew Biggs³

¹State University of New York at Plattsburgh, Plattsburgh, New York, USA.

|December 14, 2021

Summary

Genome-scale metabolic models (GEMs) offer insights into microbial ecosystems. This review explores GEM applications in microbiome research, highlighting challenges and future directions for trustworthy analysis.

Keywords:

metabolic modeling metabolism microbiome

More Related Videos

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Oct 10, 2025

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

Systems biology
Microbiome research
Metabolic modeling

Background:

Genome-scale metabolic models (GEMs) are established tools for predicting microbial phenotypes.
GEMs provide mechanistic insights into microbial ecological processes, making them valuable for microbiome research.

Purpose of the Study:

To outline opportunities for applying GEMs in microbiome research.
To present current challenges hindering trustworthy GEM application in this field.
To suggest future approaches for advancing GEM-based microbiome research.

Main Methods:

Review of existing literature on GEMs and microbiome research.
Identification of current limitations and challenges.
Proposal of strategies for future development.

Main Results:

GEMs present significant opportunities for understanding microbial communities.
Key challenges include data integration, model validation, and computational scalability.
Future work should focus on improving model accuracy and accessibility.

Conclusions:

GEMs hold great potential for advancing microbiome research.
Addressing current challenges is crucial for reliable and impactful applications.
Further development is needed to fully realize the capabilities of GEMs in this domain.