Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.0K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fifty Years after Viking: The Promise of Solar System Microbiology and Microbial Ecology.

Astrobiology·2026
Same author

Bioleaching of critical trace metals by <i>Sphingomonas desiccabilis</i>: substrate-driven selectivity in Earth and space analogues.

Frontiers in microbiology·2026
Same author

Ammonia as a parameter shaping habitability on icy moons.

FEMS microbes·2026
Same author

Diverse organic molecules on Mars revealed by the first SAM TMAH experiment.

Nature communications·2026
Same author

Distribution of extraterrestrial nucleobases, other N-heterocycles, and their precursors in a sample from asteroid Bennu.

Communications chemistry·2026
Same author

Understanding Daptomycin Resistance Mechanisms and Treatment Challenges in <i>Enterococcus faecium</i> Infection: A Case Series.

Antibiotics (Basel, Switzerland)·2026

Related Experiment Video

Updated: Oct 10, 2025

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

3.8K

Recommendation on Orbiting Sample Cleanliness.

Charles S Cockell1, Rohit Chitale2, Brian Clement3

  • 1University of Edinburgh, Centre for Astrobiology, School of Physics and Astronomy, Edinburgh, UK.

Astrobiology
|December 14, 2021
PubMed
Summary

The Mars Sample Return (MSR) campaign recommends cleaning the Orbiting Sample (OS) container to a Planetary Protection level 4a standard. This ensures sample integrity while balancing feasibility for the MSR mission.

More Related Videos

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.9K
An Ultra-clean Multilayer Apparatus for Collecting Size Fractionated Marine Plankton and Suspended Particles
09:01

An Ultra-clean Multilayer Apparatus for Collecting Size Fractionated Marine Plankton and Suspended Particles

Published on: April 19, 2018

9.0K

Related Experiment Videos

Last Updated: Oct 10, 2025

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

3.8K
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.9K
An Ultra-clean Multilayer Apparatus for Collecting Size Fractionated Marine Plankton and Suspended Particles
09:01

An Ultra-clean Multilayer Apparatus for Collecting Size Fractionated Marine Plankton and Suspended Particles

Published on: April 19, 2018

9.0K

Area of Science:

  • Planetary Science
  • Astrobiology
  • Space Mission Operations

Background:

  • The NASA-ESA Mars Sample Return (MSR) campaign aims to bring Martian samples to Earth for analysis.
  • The Orbiting Sample (OS) container is critical for sample containment during the MSR mission.
  • Maintaining stringent cleanliness within the OS is paramount for mission success and scientific integrity.

Purpose of the Study:

  • To evaluate and recommend appropriate planetary protection cleanliness levels for the MSR Orbiting Sample (OS) container.
  • To assess the trade-offs between COSPAR planetary protection cleanliness levels 4a and 4b for the OS interior.
  • To provide a consensus recommendation and rationale for the OS cleanliness requirements.

Main Methods:

  • Convened the Mars Sample Return Orbiting Sample Tiger Team (OSTT) to discuss cleanliness options.
  • Evaluated Committee on Space Research (COSPAR) planetary protection regulations, specifically levels 4a and 4b.
  • Documented the team's consensus opinion and the reasoning behind the recommendation.

Main Results:

  • The OSTT recommended cleaning the OS interior to a Planetary Protection level 4a.
  • The recommendation includes striving for any feasible additional measures towards level 4b.
  • Level 4a requires <300 bacterial spores/m² and <3 x 10⁵ bacterial spores on the spacecraft interior.

Conclusions:

  • The recommended cleanliness level (4a with efforts toward 4b) balances stringent requirements with mission feasibility.
  • This approach ensures the integrity of returned Martian samples for scientific study.
  • The decision supports the overarching goals of the Mars Sample Return campaign.