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Updated: Oct 10, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Microbial Identification in the Clinical Microbiology Laboratory Using MALDI-TOF-MS
Iván Bloise1, M Teresa Corcuera1, Julio García-Rodríguez1
1Servicio de Microbiología, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is a successful method for identifying microorganisms in clinical labs. This study details routine pathogen identification procedures using the Bruker MALDI Biotyper system.
Area of Science:
- Microbiology
- Analytical Chemistry
- Biotechnology
Background:
- Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is widely adopted in clinical microbiology.
- Its adoption is due to its simplicity, speed, and high reproducibility.
- MALDI-TOF MS has become a reference method for microorganism identification.
Purpose of the Study:
- To describe routine procedures for pathogen identification.
- To highlight the application of the Bruker MALDI Biotyper system in clinical microbiology.
Main Methods:
- Utilizing MALDI-TOF mass spectrometry.
- Employing the Bruker MALDI Biotyper system for microbial identification.
- Implementing routine laboratory protocols for pathogen analysis.
Main Results:
- MALDI-TOF MS provides successful identification of cultured microorganisms.
- The Bruker MALDI Biotyper system is effective for routine pathogen identification.
- The method is characterized by simplicity, speed, and high reproducibility.
Conclusions:
- MALDI-TOF mass spectrometry is a valuable tool in clinical microbiology.
- The Bruker MALDI Biotyper system facilitates efficient and reliable pathogen identification.
- The described procedures support the routine use of MALDI-TOF MS in diagnostic laboratories.
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