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Related Concept Videos

Subviral Agents01:29

Subviral Agents

177
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
177

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Related Experiment Video

Updated: Oct 10, 2025

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
09:20

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Published on: October 14, 2011

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Transmission Electron Microscopic Methods for Plant Virology.

Li Xie1, Jian Hong2

  • 1State Key Laboratory of Rice Biology, Analysis Center of Agrobiology and Environmental Science, Zhejiang University, Hangzhou, China. hyena_xieli@163.com.

Methods in Molecular Biology (Clifton, N.J.)
|December 14, 2021
PubMed
Summary

Transmission electron microscopy (TEM) visualizes plant viruses and infected cells. This guide details negative staining and ultrathin section methods for ultrastructural analysis.

Keywords:
CytopathologyNegative stainingPlant virusesTransmission electron microscopyUltrathin sectionVirion

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Area of Science:

  • Plant Virology
  • Microscopy Techniques

Background:

  • Transmission electron microscopy (TEM) is crucial for plant virus research.
  • Key TEM methods include negative staining and ultrathin sectioning.

Purpose of the Study:

  • To describe the operational details of negative staining and ultrathin section TEM.
  • To highlight their applications in plant virology.

Main Methods:

  • Negative staining for high-resolution virus particle structure.
  • Immuno-negative staining for virus enrichment and identification.
  • Ultrathin sectioning for host cell ultrastructure and viral infection studies.

Main Results:

  • Negative staining offers rapid sample preparation for crude sap or purified viruses.
  • Ultrathin sectioning reveals virus-induced cytopathology and subcellular distribution.
  • TEM data aids in understanding virus replication, assembly, and transport.

Conclusions:

  • Both negative staining and ultrathin section TEM are essential for comprehensive plant virus research.
  • These techniques provide insights into viral behavior and infection cycles.