Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1

B D Clark, K L Collins, M S Gandy

    Nucleic Acids Research
    |October 24, 1986
    PubMed
    Summary

    Researchers isolated the human prointerleukin 1 (proIL-1) beta gene, revealing its seven-exon structure and regulatory elements. Its similarity to the proIL-1 alpha gene suggests a duplication origin.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Improving management of needle distress during the journey to dialysis through psychological education and training-the INJECT study feasibility pilot protocol.

    Pilot and feasibility studies·2022
    Same author

    MR Imaging Features of Middle Cranial Fossa Encephaloceles and Their Associations with Epilepsy.

    AJNR. American journal of neuroradiology·2020
    Same author

    Signal recovery from stimulation artifacts in intracranial recordings with dictionary learning.

    Journal of neural engineering·2020
    Same author

    Steroid hormones, energetic state, and immunocompetence vary across reproductive contexts in a parthenogenetic lizard.

    General and comparative endocrinology·2019
    Same author

    Feeding postures of suspension-feeding larval black flies: the conflicting demands of drag and food acquisition.

    Oecologia·2017
    Same author

    Transcranial Doppler Ultrasound Detection of Microemboli as a Predictor of Cerebral Events in Patients with Symptomatic and Asymptomatic Carotid Disease: A Systematic Review and Meta-Analysis.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2016

    Area of Science:

    • Molecular Biology
    • Genetics
    • Immunology

    Background:

    • Interleukin-1 (IL-1) is a key cytokine in inflammation and immunity.
    • Understanding the genetic basis of IL-1 production is crucial for therapeutic development.

    Purpose of the Study:

    • To isolate and characterize the human prointerleukin 1 (proIL-1) beta gene.
    • To elucidate the genomic organization and regulatory features of the proIL-1 beta gene.
    • To investigate the evolutionary relationship between proIL-1 beta and proIL-1 alpha.

    Main Methods:

    • Gene isolation from human leukocyte and fetal liver DNA libraries.
    • Nucleotide sequencing to determine gene structure and regulatory elements.
    • Comparative genomic analysis with the human proIL-1 alpha gene.

    Related Experiment Videos

    Main Results:

    • Successfully isolated the human proIL-1 beta gene, comprising seven exons.
    • Identified key regulatory sequences including TATA, CAT, and poly-adenylation signals.
    • Observed significant genomic organization similarity to the human proIL-1 alpha gene.
    • Detected signatures of gene duplication, such as flanking direct repeats and a genomic poly A tail.

    Conclusions:

    • The human proIL-1 beta gene possesses a distinct exon-intron structure and regulatory elements.
    • The proIL-1 beta gene likely originated from a duplication event of the proIL-1 alpha gene, mediated by reverse transcriptase.