A simple hydrazone probe for recognition of Al³⁺ and PPi and its applicability in lysosomal imaging

A simple hydrazone probe (1) was designed and synthesized for the successive detection of Al³⁺ and pyrophosphate (PPi) in almost 100% buffer environment. The probe provided O₂N donor set for chelation with Al³⁺, leading to a distinct fluorescence boost at 510 nm. The in-situ formed 1-Al³⁺ complex detected PPi with an "on-off" behavior. The detection limits for Al³⁺ and PPi were 35.7 nM and 76 nM, respectively. Benefiting from the existence of morpholine as lysosome-targeting group, probe 1 was successfully applied to the detection of Al³⁺ and PPi in lysosomes.