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Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study
Daliah Alves Coelho Trevisan1, Paula Aline Zanetti Campanerut-Sa1, Alex Fiori da Silva2
1State University of Maringa, Department of Clinical Analysis and Biomedicine, Maringa, Brazil.
Abstract:
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.
Insights
Carvacrol disrupts Salmonella Typhimurium biofilms by altering key proteins involved in biofilm formation and antioxidant defense. This study identifies specific protein targets, offering new insights into carvacrol's antibiofilm mechanisms.
Area of Science:
- Microbiology
- Proteomics
- Bacterial Pathogenesis
Background:
- Salmonella Typhimurium biofilms pose significant challenges in healthcare and food safety.
- Carvacrol, a natural compound, exhibits known antimicrobial activity but its antibiofilm mechanisms remain unclear.
- Understanding carvacrol's effects on biofilm protein expression is crucial for developing novel anti-biofilm strategies.
Purpose of the Study:
- To investigate the impact of carvacrol treatment on the protein profile of Salmonella Typhimurium biofilms.
- To identify specific proteins affected by carvacrol that are involved in biofilm formation and antioxidant processes.
- To elucidate the molecular mechanisms underlying carvacrol's antibiofilm activity.
Main Methods:
- Comparative proteomic analysis of carvacrol-treated and untreated Salmonella Typhimurium biofilms.
- Mass spectrometry-based identification and quantification of differentially expressed proteins.
- Bioinformatic analysis to determine the functional roles of identified proteins.
Main Results:
- Carvacrol treatment led to decreased synthesis of key proteins including DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and Superoxide Dismutases (SODs) A, B, and C.
- These downregulated proteins are critically involved in Salmonella Typhimurium biofilm development and oxidative stress response.
- The proteomic data reveals significant alterations in protein expression patterns upon carvacrol exposure.
Conclusions:
- Carvacrol exerts its antibiofilm effect by downregulating essential proteins required for Salmonella Typhimurium biofilm integrity and function.
- The identified proteins represent potential targets for future therapeutic interventions aimed at combating S. Typhimurium biofilms.
- This study provides a deeper understanding of carvacrol's antibiofilm mechanism at the proteomic level.
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