Related Experiment Video
Updated: Aug 11, 2026

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Published on: June 12, 2013
Purification of human monocytes by continuous gradient sedimentation in ficoll
Abstract:
A new method of obtaining purified human monocytes has been developed. The peripheral blood mononuclear leukocytes are isolated by centrifugation over Ficoll--Hypaque and then further purified by sedimentation over a linear 5--10% Ficoll density gradient. In ten experiments, the average purity obtained was 77.1% macrophages and the mean yield was 22.4% of the monocytes contained in the peripheral blood leukocytes. Viability of monocytes isolated by this technique exceeded 95%. The cells were phagocytic and responded to human migration inhibitory factor.
More Related Videos
09:32Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Published on: September 9, 2014
05:21Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility
Published on: August 11, 2023
Related Concept Videos
Overview Of Cell Separation And Isolation
Centrifugation