Statistical analysis of a liquid phase radioimmunoassay for immune complex determination

A computer program is presented which enables iterative determination of the weighted standard curve and the quality control parameters calculation for the liquid phase radioimmunoassay which employs binding of labelled C1q as a measure of the presence of immunological complexes in serum samples. 22 independently performed laboratory tests were evaluated with respect to the presence of relationship between the dose (standardized human IgG aggregates) and response (fraction of radiolabelled C1q bound). On 14 occasions the plotted curve was sigmoidal in shape, the rest of the curves did not reach lower or upper asymptote. When the logistic transformation of this data was used the dose-response curve was linear and could be plotted with the use of the least square method. This allows calculation of the two other parameters of the sigmoidal curve (slope and midpoint). The slope of the weighted regression line, intercept point with the axis of ordinates and correlation coefficient were determined by means of the least square method for weighted linear regression with repetitions. When the iterative procedure was completed, content of immunological complexes in sera was calculated. The program is written in Fortran for the ODRA 1300 computer series. The printout consist of three parts. First part contains the original data, parameters and information about the form of input data. The second part of the printout consist of the results, obtained from non-weighted and weighted regression lines and the plotted regression line. The third part of the printout contains the final results of immune complexes determination.