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Updated: Oct 10, 2025

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
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CD45 Immunohistochemistry in Mouse Kidney.

Shirong Zheng1, Paul N Epstein2

  • 1Diabetes and Obesity Center, Division of Environmental Medicine, School of Medicine, University of Louisville, KY, USA.

Bio-Protocol
|December 15, 2021
PubMed
Summary
This summary is machine-generated.

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This study presents a reliable immunohistochemistry protocol for CD45 staining in mouse kidney tissue. The method effectively identifies inflammatory cells, aiding in the study of kidney injury and diseases like diabetes.

Area of Science:

  • Immunohistochemistry
  • Cell Biology
  • Pathology

Background:

  • CD45 is a crucial pan-leukocyte marker used to assess inflammatory cell infiltration and its link to tissue damage.
  • Standardized protocols are essential for accurate CD45 staining in research settings.

Purpose of the Study:

  • To establish and validate a reliable immunohistochemistry (IHC) protocol for CD45 staining in paraffin-embedded mouse kidney sections.
  • To demonstrate the utility of this protocol in identifying inflammatory cells in kidney injury models.

Main Methods:

  • Utilized a rat anti-CD45 antibody as the primary antibody.
  • Employed a biotin-conjugated goat anti-rat IgG secondary antibody.
  • Implemented a horseradish peroxidase (HRP)-linked avidin/biotin detection system with 3,3'-Diaminobenzidine (DAB) for signal visualization.
Keywords:
CD45DiabetesIHCKIM-1KidneyOVE

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Main Results:

  • The protocol successfully identified hematolymphoid cells in bone marrow and monocyte/macrophages in liver and lung tissues.
  • Significantly increased CD45-positive cells were observed in the kidneys of diabetic mice, validating the protocol's utility.
  • Double staining revealed CD45-positive cells accumulating around injured tubular cells and overlapping with other inflammatory cells like macrophages.

Conclusions:

  • This validated IHC protocol provides a robust method for CD45 staining in mouse kidney tissue.
  • The protocol is effective for quantifying inflammatory cell infiltration in various tissues and disease models, particularly in kidney injury research.
  • The findings highlight the importance of CD45 staining in understanding inflammatory responses in pathological conditions.