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Generation of Human Alloantigen-specific T Cells from Peripheral Blood
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Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable

H S Chou, M A Behlke, S A Godambe

    The EMBO Journal
    |September 1, 1986
    PubMed

    Both cDNA and genomic clones of the T cell receptor (TCR) alpha- and beta-chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non-functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six-member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7-14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general.

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