Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

6.2K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
6.2K
Pigmentation01:19

Pigmentation

3.3K
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
3.3K
Cells of the Epidermis01:24

Cells of the Epidermis

5.4K
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
5.4K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

4.0K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tie2-enriched nucleus pulposus cell transplantation enables severity-independent disc repair in a canine model with induced and controlled disc degeneration severity.

NPJ Regenerative medicine·2026
Same author

Extent of intramedullary short tau inversion recovery signal change predicts traumatic cervical spinal cord injury outcomes in surgically treated older adults: a multicenter study in Japan.

Asian spine journal·2026
Same author

Lost in Translation: Why Biologic Therapies for Intervertebral Disc Degeneration and Low Back Pain Have Not Reached the Clinic (Yet): Bridging Biology, Pain Phenotyping, and Trial Design.

JOR spine·2026
Same author

Celebrating ISSLS Science, Leadership, Legacy, and Partnership with the European Spine Journal.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Sacral positioning and interface pressure in the beach-chair position during shoulder surgery: a retrospective, observational study.

Journal of tissue viability·2026
Same author

Role of paraspinal muscle fatty infiltration in predicting adjacent segment disease after lumbar spinal fusion: a systematic review and meta-analysis.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026

Related Experiment Video

Updated: Oct 10, 2025

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
12:37

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Published on: September 7, 2013

18.4K

MC5R Contributes to Sensitivity to UVB Waves and Barrier Function in Mouse Epidermis.

Akari Shintani1, Hiromi Sakata-Haga1, Keiichi Moriguchi2

  • 1Department of Anatomy, School of Medicine, Kanazawa Medical University, Uchinada, Japan.

JID Innovations : Skin Science From Molecules to Population Health
|December 15, 2021
PubMed
Summary

Melanocortin 5 receptor (MC5R) deficiency increases skin sensitivity to UVB radiation and impairs barrier function. MC5R-deficient mice exhibit more severe UVB-induced skin damage and inflammation.

Keywords:
KC, keratinocyteSG, stratum granulosumTEM, transmission electron microscopyTEWL, transepidermal water lossTGN, trans-Golgi networkTLC, thin-layer chromatographyWT, wild type

More Related Videos

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

31.8K
Studying Chronic Exposure of Mice to Ultraviolet B Radiation
03:20

Studying Chronic Exposure of Mice to Ultraviolet B Radiation

Published on: August 19, 2025

1.2K

Related Experiment Videos

Last Updated: Oct 10, 2025

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
12:37

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Published on: September 7, 2013

18.4K
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

31.8K
Studying Chronic Exposure of Mice to Ultraviolet B Radiation
03:20

Studying Chronic Exposure of Mice to Ultraviolet B Radiation

Published on: August 19, 2025

1.2K

Area of Science:

  • Dermatology
  • Molecular Biology
  • Skin Physiology

Background:

  • The melanocortin 5 receptor (MC5R) is primarily known for its role in sebaceous gland function.
  • Its specific functions within the epidermis and its impact on skin homeostasis are not fully understood.

Purpose of the Study:

  • To investigate the role of MC5R in epidermal homeostasis.
  • To determine the effect of MC5R deficiency on skin responses to ultraviolet B (UVB) irradiation.

Main Methods:

  • Comparison of UVB-induced skin damage and inflammation in MC5R-deficient mice and wild-type mice.
  • Assessment of transepidermal water loss (TEWL) and epidermal barrier function.
  • Histological and ultrastructural analysis of skin tissues.
  • Measurement of inflammatory cytokine levels (IL-6, IL-10).

Main Results:

  • MC5R-deficient mice showed increased sensitivity to UVB, developing ulcers and inflammation at lower doses than wild-type mice.
  • Significantly higher transepidermal water loss was observed in MC5R-deficient mice.
  • UVB-irradiated MC5R-deficient skin exhibited increased inflammatory cell infiltration, epidermal thickening, and altered cellular composition.
  • Ultrastructural analysis revealed fewer lamellar granules and reduced lipid secretion in MC5R-deficient epidermis.

Conclusions:

  • MC5R plays a crucial role in maintaining epidermal homeostasis and skin barrier function.
  • MC5R deficiency leads to heightened susceptibility to UVB-induced skin damage and impaired barrier integrity.