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Updated: Oct 9, 2025
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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
Published on: July 17, 2014
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Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development
Nishanthi Mathiyalagan1, Sebastian Dworkin2
1Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2021
Abstract:
Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.
