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Updated: Oct 9, 2025

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
Published on: November 8, 2014
The zebrafish issue: 25 years on
Mary C Mullins1, Joaquín Navajas Acedo2, Rashmi Priya3
1University of Pennsylvania Perelman School of Medicine, Department of Cell and Developmental Biology, Philadelphia, PA 19104, USA.
Abstract:
In the 1990s, labs on both sides of the Atlantic performed the largest genetic mutagenesis screen at that time using an emerging model organism: the zebrafish. Led by Christiane Nüsslein-Volhard in Tübingen, Germany, and Wolfgang Driever in Boston, USA, these colossal screens culminated in 1996 with the publication of 37 articles in a special issue of Development, which remains the journal's largest issue to this day. To celebrate the anniversary of the zebrafish issue and reflect on the 25 years since its publication, five zebrafish researchers share what the issue means to them, how it has contributed to their career and its impact on the zebrafish community.
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