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[Gene expression profiling of testicular seminoma via bioinformatical analysis]
Ping Jiang1,2, Lun-Bin Xia1, Zhi-Mei Zhong1
1School of Biological and Pharmaceutical Engineering, West Anhui College, Lu'an, Anhui 237012, China.
Objective:
To investigate the differentially expressed genes related to the survival of testicular seminoma patients and the pathogenesis of the malignancy.
Methods:
We obtained the gene expression profiling of testicular seminoma data set GSE8607 from the GEO database and differentially expressed genes in testicular seminoma using the GEO2R online tool. We performed GO and KEGG pathway enrichment via the DAVID website, screened testicular seminoma-related core genes using the STRING and Cytoscape software, and analyzed the survival rate and expression level associated with the core genes through the Kaplan Meier plotter and GEPIA websites.
Results:
Totally, 591 differentially expressed genes were identified, of which 216 were up-regulated and mainly enriched in 23 biological processes, 8 cell components, 5 biological functions and 7 KEGG pathways, and the other 375 down-regulated and enriched only in the biological process, involving the formation and development of spermatozoa. Thirty-two core genes were obtained via PPI network analysis, including C1QB, CCL2, CCR2, GPR183, ELL, LAPTM5, NPY5R, CD28 and MMP9, which were closely related to the survival rate (P < 0.05), C1QB, CCL2, CCR2, SELL, LAPTM5, CD28 and MMP9, which were highly expressed in the testicular seminoma tissue (P < 0.05), and NPY5R, which was lowly expressed in the testicular seminoma tissue (P < 0.05).
Conclusions:
The 8 differentially expressed genes identified in the testicular seminoma tissue contribute to effective prediction of the survival rate of the patients as well as to the diagnosis, prevention and treatment of the disease and development of new anti-cancer drugs.
