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Updated: Oct 9, 2025

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
Published on: March 6, 2018
Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect
R C M Vulders1, R C van Hoogenhuizen2, E van der Giessen3
1Philips Research Laboratories, Eindhoven, The Netherlands.
Abstract:
The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.
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