Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium and exocytosis.

D E Knight

    Ciba Foundation Symposium
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Calcium and MgATP are essential for amine secretion in bovine chromaffin cells and human platelets. Guanine nucleotides like GTP and GTP gamma S modulate this secretion, suggesting dual action sites impacting cellular responses.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Secretion from bovine chromaffin cells acutely expressing exogenous proteins using a recombinant Semliki Forest virus containing an EGFP reporter.

    Molecular and cellular neurosciences·2000
    Same author

    A novel use of differential equations to fit exponential functions to experimental data.

    Journal of neuroscience methods·1994
    Same author

    Triggered exocytosis and endocytosis have different requirements for calcium and nucleotides in permeabilized bovine chromaffin cells.

    The Journal of membrane biology·1993
    Same author

    Growth factor expression during rat development: a comparison of TGF-beta 3, TGF-alpha, bFGF, PDGF and PDGF-R.

    International journal of experimental pathology·1993
    Same author

    Electropermeabilized platelets: a preparation to study exocytosis.

    Methods in enzymology·1993
    Same author

    A study of the bovine adrenal chromaffin nicotinic receptor using patch clamp and concentration-jump techniques.

    The Journal of physiology·1992
    Same journal

    Precision agriculture: spatial and temporal variability of environmental quality. General reflections.

    Ciba Foundation symposium·1997
    Same journal

    Uncertainty in hydrogeological modelling.

    Ciba Foundation symposium·1997
    Same journal

    Optimal mapping of site-specific multivariate soil properties.

    Ciba Foundation symposium·1997
    Same journal

    Modelling for precision weed management.

    Ciba Foundation symposium·1997
    Same journal

    GIS support for precision agriculture: problems and possibilities.

    Ciba Foundation symposium·1997
    Same journal

    Spatial sampling.

    Ciba Foundation symposium·1997
    See all related articles

    Area of Science:

    • Cell biology
    • Neuroscience
    • Biochemistry

    Background:

    • Amine secretion is a critical process in both neuronal and platelet function.
    • This secretion is known to be regulated by intracellular calcium and ATP.
    • The specific roles of guanine nucleotides in these secretory pathways require further elucidation.

    Purpose of the Study:

    • To investigate the role of guanine nucleotides in Ca2+-dependent amine secretion.
    • To explore the differential effects of GTP and non-hydrolyzable GTP gamma S on secretion in chromaffin cells and platelets.
    • To identify potential sites of action for guanine nucleotides in the secretory cascade.

    Main Methods:

    • Utilizing electropermeabilized bovine chromaffin cells and human platelets.
    • Measuring amine secretion in response to varying Ca2+ and MgATP concentrations.

    Related Experiment Videos

  • Assessing the impact of protein kinase C activators, thrombin, GTP, and GTP gamma S on secretory responses.

    • Main Results:

    • Ca2+ and MgATP are required for amine secretion in both cell types.
    • Activators of protein kinase C enhance Ca2+ sensitivity of secretion.
    • GTP potentiates thrombin-induced platelet secretion, while GTP gamma S inhibits chromaffin cell secretion, with evidence of dual nucleotide action sites.

    Conclusions:

    • Guanine nucleotides play a complex regulatory role in amine secretion.
    • Evidence suggests distinct mechanisms of action for GTP and GTP gamma S.
    • These findings point to at least two sites where guanine nucleotides can modulate cellular secretion, potentially involving agonist receptors and protein kinase C.