Generation of mixed murine organoids to model cellular interactions
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Published on: October 7, 2020
Ana Krotenberg Garcia1, Jacco van Rheenen1, Saskia Jacoba Elisabeth Suijkerbuijk1
1Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands.
Abstract:
Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).
