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In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
Published on: August 17, 2010
Xenograft Models of Ovarian Cancer for Therapy Evaluation
Mihaela Popa1, Vibeke Fosse1, Katrin Kleinmanns1
1CCBIO, Department of Clinical Science, University of Bergen, Bergen, Norway.
Abstract:
The evaluation of novel treatment regimes in ovarian cancer, ranging from cytotoxic agents and targeted therapy to surgery, demands clinically relevant mouse models to mimic human disease. These more advanced preclinical models provide a tool to obtain robust data on the mechanism of action, cytotoxicity and therapeutic efficacy of newly emerging antitumor therapies.In this chapter, we describe how to generate ovarian cancer xenograft models through injection of human tumor cell lines in immunocompromised mice. Detailed methodological descriptions are provided for both the commonly applied subcutaneous model and the more technically challenging orthotopic tumor model that involves inoculation of cancer cells in the ovarian bursa. We demonstrate how to monitor tumor growth and metastases in orthotopic ovarian models through noninvasive optical imaging and the procedures for treatment strategy, including administration of test compounds and debulking surgery. We comment on the strengths, limitations, and procedural challenges associated with each of the models.
Insights
Generating ovarian cancer xenograft mouse models is crucial for evaluating new treatments. This chapter details subcutaneous and orthotopic methods for preclinical studies of ovarian cancer therapies.
Area of Science:
- Oncology
- Preclinical Research
- Animal Models
Background:
- Evaluating novel ovarian cancer treatments requires clinically relevant preclinical models.
- Mouse models are essential for assessing the efficacy and mechanism of action of emerging antitumor therapies.
Purpose of the Study:
- To describe the generation and utilization of ovarian cancer xenograft mouse models.
- To provide detailed methods for both subcutaneous and orthotopic tumor models.
- To outline monitoring, treatment strategies, and discuss model limitations.
Main Methods:
- Generation of ovarian cancer xenograft models via injection of human tumor cell lines into immunocompromised mice.
- Detailed protocols for subcutaneous and orthotopic (ovarian bursa inoculation) models.
- Noninvasive optical imaging for monitoring tumor growth and metastases; administration of test compounds and surgical debulking procedures.
Main Results:
- Successful establishment of both subcutaneous and orthotopic ovarian cancer xenograft models.
- Demonstration of tumor growth monitoring and therapeutic intervention capabilities.
- Comparative analysis of the strengths, limitations, and challenges of each model.
Conclusions:
- Ovarian cancer xenograft mouse models are vital tools for preclinical evaluation of novel therapeutic strategies.
- Both subcutaneous and orthotopic models offer distinct advantages for studying ovarian cancer progression and treatment response.
- Detailed methodological insights are provided to facilitate the application and optimization of these models in research.
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